KMID : 0525720110160040243
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Journal of Chitin and Chitosan 2011 Volume.16 No. 4 p.243 ~ p.248
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Biological Characteristics of Chitinolytic Fungus Penicillium janthinellum LPB-01
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Koo Bon-Geun
Baek Gu Shin Seo-Yoon Kim Min-Jung Kim Tae-Jun Seon Jun-Hong Jeon So-Young Im Da-Young Park Ji-Min Park Jae-Kweon
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Abstract
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The aim of the present study is to define the characteristics and biological activities of chitinolytic fungus Penicillium janthinellum LPB-01. Chitinolytic activity was determined using 4-nitrophenyl N-acetyl-¥â-D-glucosaminide (about 417 pmol/ ¥ìg/min) and 4-Nitrophenyl N,N`-diacetyl-¥â-D-chitobiosides (665 pmol/¥ìg/min) as substrates, and reducing sugar assay. P. janthinellum LPB-01 actively degrades chitin and sulfated chitin producing chitooligosacharides. These results revealed that P. janthinellum LPB-01 is suitable for depolymerizing chitinous materials including sulfated chitin to produce low molecular weight chitooligosacharides. In addition, significant anti-oxidant effects of culture supernatant of P. janthinellum LPB-01 cultured in different conditions were determined by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical. Based on these results, we concluded that P. janthinellum LPB-01 can be used as a produced for potentially active materials.
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KEYWORD
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chitinolytic enzyme, chitin, chitooligosaccharides, Penicillium janthinellum, fungi
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